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本文介绍了定性定量分析大麻的主要代谢物ＴＨＣ－ＣＯＯＨ的两种方法。所有试管硅烷化，尿样碱性水解。ＴＬＣ法快速、简便，适用于多份检材的同时定性分析，最低检测限为５０ｎｇ．采用衍生化、ＳＩＭ的ＧＣ／ＭＳ方法灵敏，选择性、专一性强．五氟丙酸酐衍生化方法线性范围为２０－２５０ｎｇ／ｍｌ，最低检测限１０ｎｇ／ｍｌ，变异系数为８．４％，提取回收率为５８．９％；甲基化方法线性范围１０～１００ｎｇ／ｍｌ，最低检测限５ｎｇ／ｍｌ，变异系数为５．６％，提取回收率为５３．２％．
This article presents two methods for the qualitative and quantitative analysis of THC-COOH, the major metabolite of cannabis. All tubes were silanized and the urine sample was alkaline hydrolyzed. TLC method is fast and simple, suitable for simultaneous qualitative analysis of multiple samples, the minimum detection limit is 50ng. Derivatization, SIM GC / MS method is sensitive, selective and specific. The linear range of derivatization of pentafluoropropionic anhydride was 20-250ng / ml, the lowest detection limit was 10ng / ml, the coefficient of variation was 8.4% and the recovery rate was 58.9%. The linear range of methylation was 10 ~ 100ng / ml, the lowest detection limit was 5ng / ml, the coefficient of variation was 5.6% and the recovery rate was 53.2%.