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基于同源序列克隆技术,从花椰菜中克隆了查尔酮合酶(chalcone synthase;CHS)基因cDNA全长,将该基因成功转入花椰菜,经过Basta抗性筛选,PCR鉴定,得到7株花椰菜转基因过表达阳性植株.转基因花椰菜的核盘菌胁迫分析显示,与对照花椰菜相比,在病原胁迫的不同时期,转基因植株中BoCHS基因的表达量显著上升,且随着胁迫时间的增加,该基因表达量均增高,且在36 h时达到最大值,表明转基因花椰菜通过过量表达CHS来增强自身对菌核病的抗性,使植株对菌核病的抗性明显增强.与查尔酮合酶在油菜、向日葵、烟草等中的抗病作用一致,表明花椰菜BoCHS基因也是抗菌核病的重要基因.
Based on the homologous sequence cloning technology, the full length cDNA of chalcone synthase (CHS) gene was cloned from cauliflower. The gene was successfully transferred into cauliflower. After Basta resistance screening and PCR identification, seven cauliflower transgenic Overexpression of positive plants.The analysis of S. sclerotiorum in S. cauliflower showed that the expression of BoCHS gene in transgenic plants increased significantly at different stages of pathogen stress compared with control cauliflower and the expression of this gene was increased with the increase of stress time And reached the maximum at 36 h, indicating that transgenic cauliflower enhanced its resistance to sclerotinia by overexpression of CHS, and the resistance of plants to sclerotinia was significantly enhanced.Compared with chalcone synthase in Rape, sunflower, tobacco and other resistance role in the same, indicating that the cauliflower BoCHS gene is also an important gene of anti-sclerotinia.