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目的比较吡柔比星和其他化疗药物的对胃癌组织温热协同作用,探讨化疗药物与温热化疗的协同作用机制。方法利用36个病例的手术切除胃癌组织,建立人胃癌组织体外立体培养作用模型,MTS-PMS比色法检测组织培养物活性以了解化疗药物以及温热化疗的抑瘤效果。HE染色研究评价药物对胃癌组织在组织形态学上的改变。结果顺铂、丝裂霉素和吡柔比星对胃癌组织的杀伤均具有较强的温热协同关系(P=0.000),吡柔比星单独处理和温热协同处理均具有较强的抑制作用,和其他3种药物抑制率比较具有显著性差异(P<0.05)。吡柔比星和温热协同处理效果与胃癌病例的临床分期和组织学分级有关(P=0.000),而与年龄、性别、肿瘤大小及术前CEA水平无关(P>0.05)。HE染色表明,吡柔比星和温热处理后,胃癌组织大部崩解,细胞变性坏死,细胞核固缩,胞浆散失。结论THP对胃癌组织具有良好的温热协同效应和肿瘤杀伤效果,具有进一步临床研究价值。
Objective To compare the synergistic effects of pirarubicin and other chemotherapeutic drugs on gastric cancer tissue and to explore the synergistic mechanism of chemotherapeutic drugs and hyperthermia chemotherapy. Methods Thirty-six cases of gastric cancer tissues were resected to establish a model of human gastric cancer cells in vitro. MTS-PMS assay was used to detect the activity of tissue culture in order to understand the anti-tumor effect of chemotherapy drugs and warm chemotherapy. Hematoxylin and eosin (HE) staining was used to assess the histomorphological changes of gastric cancer. Results Cisplatin, mitomycin and pirarubicin had a strong synergistic effect on the gastric cancer (P = 0.000). Both pirarubicin alone and warm synergistic treatment showed a strong inhibition There was significant difference (P <0.05) between the inhibition rate and other three drugs. The effect of pirarubicin and warm synergistic treatment was related to the clinical stage and histological grade of gastric cancer (P = 0.000), but not to age, sex, tumor size and preoperative CEA level (P> 0.05). HE staining showed that pirarubicin and warm treatment, most of the disintegration of gastric cancer tissue, cell degeneration and necrosis, nuclear pyknosis, cytoplasm loss. Conclusion THP has a good warm synergistic effect and tumor killing effect on gastric cancer tissue, and has further clinical research value.