论文部分内容阅读
目的:研究基质金属蛋白酶-2(MMP-2)和组织基质金属蛋白酶抑制剂-2(TIMP-2)在人肺癌组织中的表达及其与微血管密度(MVD)的关系。方法:免疫组织化学方法检测90例肺癌组织和40例癌周组织中MMP-2和TIMP-2的表达,Ⅷ因子染色计数局部MVD,分析MMP-2和TIMP-2与MVD的关系。结果:免疫组织化学染色结果经IPP软件分析显示,肺癌组织MMP-2的表达值高于癌周组织(P<0.001),腺癌高于鳞癌(P<0.05),小细胞癌最高(P<0.05),并发淋巴结转移的肺癌组织高于无转移组(P<0.001);肺癌组织TIMP-2的表达低于癌周肺组织(P<0.001),在各种不同病理类型肺癌中的表达差异无显著性(P>0.05),并发淋巴结转移的肺癌组织表达较低(P<0.05)。MMP-2表达强度随着MVD的增高而增强,TIMP-2表达程度随着MVD的增高而减弱(P<0.05)。结论:MMP-2在各种不同病理类型的肺癌组织中有不同程度的表达,MMP-2和TIMP-2的表达水平与肺癌局部的MVD有关。
Objective: To investigate the expression of matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of matrix metalloproteinase-2 (TIMP-2) in human lung cancer and its relationship with microvessel density (MVD). Methods: The expressions of MMP-2 and TIMP-2 in 90 specimens of lung cancer and 40 specimens of peritumoral tissue were detected by immunohistochemistry. The local MVD was counted by factor Ⅷ staining. The relationship between MMP-2, TIMP-2 and MVD was analyzed. Results: The results of immunohistochemistry showed that the expression of MMP-2 in lung cancer was higher than that in peri-cancerous tissues (P <0.001) by IPP software, higher in adenocarcinoma than in squamous cell carcinoma (P <0.05) and in small cell carcinoma (P <0.001). The expression of TIMP-2 in lung cancer tissues was lower than that in lung cancer tissues (P <0.001), and the expression in different pathological types of lung cancer There was no significant difference between the two groups (P> 0.05). The expression of LN in lung cancer with lymph node metastasis was lower (P <0.05). The expression of MMP-2 increased with the increase of MVD, while the expression of TIMP-2 decreased with the increase of MVD (P <0.05). Conclusions: MMP-2 is expressed in different degree in different pathological types of lung cancer tissues. The expression of MMP-2 and TIMP-2 is correlated with MVD in lung cancer.