目前,疟疾在热带和亚热带地区的一些国家仍然猖獗流行。据推测,在热带地区约有两亿人受到疟疾的严重威胁,仅非洲每年就有九千六百万疟疾病例,死亡者多达一百万人,主要是受染的儿童。在疟疾防治研究中,鉴于疟原虫和媒介蚊虫抗性株的出现,积极探索疟疾主动免疫的方法来完善现有的抗疟措施是一个重要课题。自从1975年Kohler和Milstein研究淋巴细胞杂交瘤技术成功之后,对疟疾疫苗的研究起到巨大的推动作用并使其得到迅速的发展。关于鸟疟、鼠疟、猴疟和人疟等多种疟原虫的子孢子期或是裂殖子和配子期的单克隆抗体(McAb)都已有比较深入的研究。在国内,高敏新等和李文渌等,已对恶性疟原虫和约氏疟原虫建成MCAb株。在细胞融合方法中,一般采用BALB/c小鼠脾细胞与小鼠骨髓瘤细胞融合。但在诺氏疟原虫MCAb的研究中,应用Y_3大鼠骨髓瘤细胞同Ao大鼠脾细胞融合,也获得成功。为了生产人单克隆抗体,Monjour等 Currently, malaria is still prevalent in some countries in the tropics and subtropics. Presumably, some 200 million people in the tropics are seriously threatened by malaria, with 96 million cases of malaria each year in Africa and up to one million deaths, mainly of infected children. In the study of malaria control, in view of the emergence of resistant strains of malaria and vector-borne mosquitoes, it is an important issue to actively explore malaria active immunization methods to improve the existing antimalarial measures. Since 1975, Kohler and Milstein study of lymphocyte hybridoma technology success, the study of malaria vaccine play a huge role in promoting and rapid development. There have been more studies on the sporozoite or merozoite and gametogenic McAbs of Plasmodium such as avian malaria, murine malaria, malaria and human malaria. In China, Gao Minxin et al. And Li Wenlian et al. Have constructed MCAb strains for Plasmodium falciparum and Plasmodium yoelii. In the cell fusion method, BALB / c mouse spleen cells are generally fused with mouse myeloma cells. However, in the study of Plasmodium knowlesi MCAb, Y_3 rat myeloma cells were also fused with Ao rat splenocytes and successfully. For the production of human monoclonal antibodies, Monjour et al