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目的 :研究口腔疣状癌中基质金属蛋白酶 (MMP 2 )的表达 ,探讨其不同生物学行为的分子基础。方法 :15例口腔疣状癌标本 ,10例正常口腔黏膜标本 ,2 0例口腔鳞癌 (高、低分化鳞癌各 10例 )标本 ,应用免疫组织化学S P法检测上述标本MMP 2表达和分布。结果 :口腔疣状癌和口腔鳞癌中MMP 2主要表达于癌细胞胞浆 ,正常口腔黏膜MMP 2阴性表达。口腔疣状癌MMP 2阳性表达率为 3 3 .3 % (5 /15 ) ,平均染色强度低于高分化鳞癌和低分化鳞癌组 (P <0 .0 5 )。口腔疣状癌、口腔高分化鳞癌、口腔低分化鳞癌MMP 2表达均高于正常口腔黏膜组(P <0 .0 5 )。结论 :口腔疣状癌细胞产生的MMP 2 ,导致基底膜成分Ⅳ型胶原降解 ,破坏基底膜的完整性 ,这可能是口腔疣状癌局部侵袭转移的机制之一。MMP 2在口腔疣状癌、口腔高分化鳞癌、口腔低分化鳞癌的表达存在明显差异 ,证实口腔疣状癌是一种独立类型的恶性肿瘤。
Objective: To study the expression of matrix metalloproteinase (MMP 2) in oral verrucous carcinoma and to explore the molecular basis of different biological behaviors. Methods: 15 cases of oral verrucous carcinoma specimens, 10 normal oral mucosa specimens and 20 oral squamous cell carcinomas (10 cases of high and poorly differentiated squamous cell carcinoma) specimens were collected. The expression and distribution of MMP-2 in these specimens were detected by immunohistochemical SP method . Results: The expression of MMP-2 in oral wart carcinoma and oral squamous cell carcinoma was mainly expressed in cytoplasm of cancer cells, but negative in normal oral mucosa. The positive expression rate of MMP 2 in oral verrucous carcinoma was 33.3% (5/15), and the average staining intensity was lower than that in well differentiated squamous cell carcinoma and poorly differentiated squamous cell carcinoma (P <0.05). Oral verrucous carcinoma, well-differentiated squamous cell carcinoma and poorly differentiated squamous cell carcinoma of oral mucosa have higher MMP-2 expression than normal oral mucosa (P <0.05). CONCLUSION: MMP2 produced by oral verrucous carcinoma cells leads to the degradation of type IV collagen in basement membrane and the destruction of integrity of basement membrane, which may be one of the mechanisms of local invasion and metastasis of oral verrucous carcinoma. The expression of MMP-2 in oral verrucous carcinoma, well-differentiated squamous cell carcinoma and poorly differentiated oral squamous cell carcinoma of the oral cavity is significantly different, confirming that oral verrucous carcinoma is an independent type of malignant tumor.