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应用植物血凝素(PHA)和脂多糖(LPS)激活淋巴细胞,以氢-胸腺嘧啶核苷(~3H-TdR)、碳一尿嘧啶核苷(~(14)C-UR)和碳-缬氨酸作掺入实验,以分别反映T、B淋巴细胞转化过程中的DNA、RNA和蛋白质的合成能力。共测定了50例肿瘤病人,与正常人比较:T淋巴细胞转化能力明显降低,B淋巴细胞转化能力显著增高。经过一个疗程的~(60)Co照射后T细胞转化比照前又显著降低,B细胞转化比照前又显著增高,三种大分子合成都表现同样的规律,反映了辐射抑制T淋巴细胞DNA和RNA合成,相反地刺激了B淋巴细胞的DNA、RNA和蛋白质的合成。以上的辐射效应随照射剂量和照射野的增加而愈益明显。
Activation of lymphocytes with phytohemagglutinin (PHA) and lipopolysaccharide (LPS) with hydrogen-thymidine (~3H-TdR), carbon-uridine (14C-UR) and carbon- Proline was used as an incorporation experiment to reflect the ability of DNA, RNA and protein synthesis in the transformation of T and B lymphocytes, respectively. A total of 50 tumor patients were measured and compared with normal subjects: T lymphocyte transformation ability was significantly reduced, and B lymphocyte transformation capacity was significantly increased. After a course of ~(60)Co irradiation, the T cell transformation was significantly reduced compared to before, and the B cell transformation was significantly higher than before. The three macromolecules all showed the same pattern, reflecting the radiation-suppressing T lymphocyte DNA and RNA. Synthesis, on the contrary, stimulates the synthesis of DNA, RNA and proteins of B lymphocytes. The above radiation effects become more and more obvious with the increase of irradiation dose and irradiation field.