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猪胆汁酸钠不仅是人工牛黄的主要成分,而且有广泛的临床用途.近年来,文献报道已经在实验大鼠证实口服少量牛磺胆酸可以克服长期摄入维生素A酸(retinoic acid,RA)或其衍生物所引起的副作用.鉴于RA是髓系白血病细胞分化的生理性诱导剂,临床试用表明口服RA对急性早幼粒白血病有较满意疗效,我们设想胆汁酸与RA联合用药可能对早幼粒白血病的治疗是有意义的.本文报道体外试验结果表明,药用猪汁酸钠明显抑制HL-60细胞增殖(IC_(50) 400μg/ml)并诱导HL-60细胞向终末方向分化.诱导后的HL-60细胞具有嗜中性粒细胞和单核/巨噬细胞的某些形态及细胞化学特征,表现出明显的细胞呼吸爆发功能.细胞周期分析表明猪胆汁酸钠阻断细胞从G_0+G_1期进入S期.小鼠急性中毒试验测定其LD_(50)为:腹腔注射LD_(50)462±SD 35 mg/kg,灌胃LD_(50)>1 g/kg。药用猪胆酸钠体外试验对粒系白血病原代细胞的增殖抑制及分化诱导作用以及体内试验对某些小鼠白血病的作用正在进一步观察.我们认为进一步研究猪胆汁酸钠和RA的联合应用是有意义的.
Pig bile sodium is not only the main component of artificial bezoar, but also has a wide range of clinical applications.In recent years, it has been reported in the literature that a small amount of taurocholic acid can overcome long-term intake of retinoic acid (RA) Or derivatives thereof.As RA is a physiological inducer of myeloid leukemic cell differentiation, clinical trials have shown that oral RA is more satisfactory in acute promyelocytic leukemia, and we hypothesized that the combination of bile acid and RA may have a positive effect on early The treatment of leukemia is meaningful.In this paper, we report in vitro test results showed that the medicinal pigs sabotin significantly inhibited the proliferation of HL-60 cells (IC 50 (400μg / ml)) and induced HL-60 cells to the terminal direction of differentiation .The induced HL-60 cells have certain morphological and cytochemical characteristics of neutrophils and monocytes / macrophages, and showed obvious cell respiratory burst function.Peripal cycle analysis showed that porcine bile acid block cells From G_0 + G_1 to S phase, LD_ (50) was determined by acute poisoning test in mice: intraperitoneal injection of LD_ (50) 462 ± SD 35 mg / kg and LD_ (50)> 1 g / kg. The effects of medicinal sodium cholate in vitro on the proliferation inhibition and differentiation induction of myeloid leukemia primary cells and in vivo effects on some mouse leukemias are under further observation.We consider further study on the combination of porcine bile sodium and RA meaningful.