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A polymerized liposome (PLS) was prepared using a synthesized phospholipid with a diacetylene moiety.The precipitable property of the PLS on salt additions,ligand immobilization,affinity adsorption kinetics and adsorption isotherms were studied.Trypsin was purified by affinity precipitation from a crude pancreatic extract.The specific activity reached about 13000 U.mg~(－1). These results indicate that the PLS is a potential ligand carrier for affinity precipitation of proteins.
A polymerized liposome (PLS) was prepared using a synthesized phospholipid with a diacetylene moiety. The precipitable property of the PLS on salt additions, ligand immobilization, affinity adsorption kinetics and adsorption isotherms were studied. Triptin was purified by affinity precipitation from a crude pancreatic extract The specific activity reached about 13000 U.mg ~ (-1). These results indicate that the PLS is a potential ligand carrier for affinity precipitation of proteins.