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应用溴化乙锭染色的非同位素ＤＮＡ单链构型多态性分析新技术，对１６例卵巢癌及５例正常胎盘组织中Ｐ￣（５３）基因的第５、６外显子（Ｅｘｏｎ５、６）的聚合酶链反应扩增产物进行分析，并结合ＤＮＡ测序技术进行验证。结果表明正常胎盘组织均未发现异常，３／１６例卵巢癌ＳＳＣＰ分析有异常，ＤＮＡ测序证实这３例基因改变中有２例为核苷酸突变，另１例为核苷酸插入。采用ＥＢ染色的非同位素ＰＣＲ—ＳＳＣＰ，避免了常规ＳＳＣＰ使用放射性同位素的缺点，具有良好的实用性和广泛的应用价值。
A novel technique for non-isotopic DNA single-stranded conformation polymorphism analysis using ethidium bromide staining for exon 5, exon 5 of the P ̄ (53) gene in 16 cases of ovarian cancer and 5 cases of normal placental tissue. 6) Polymerase chain reaction amplification products were analyzed and validated by DNA sequencing techniques. The results showed that no abnormalities were found in normal placenta tissues. 3/16 cases of ovarian cancer had abnormal SSCP analysis. DNA sequencing confirmed that 2 of the 3 gene mutations were nucleotide mutations, and the other one was a nucleotide insertion. The use of non-isotopic PCR-SSCP with EB staining avoids the disadvantages of conventional SSCP using radioisotopes, and has good practicality and wide application value.