采用ＡＢＣ和ＰＣＲ－ＳＳＣＰ法在４８例ＮＨＬ中研究ｐ５３基因外显子５～８中发生突变的类型、频率与突变型ｐ５３蛋白的表达以及它们与病理分型、恶性程度的相关性。结果：突变型ｐ５３蛋白在ＮＨＬ中的阳性率为４１．７％，其中在弥漫型大细胞性淋巴瘤（ＤＬＣＬ）为７５％，在非ＤＬＣＬ为３５％，两者差异显著（Ｐ＜０．０５）；在ｐ５３蛋白表达阳性的ＮＨＬ中ＰＣＲＳＳＣＰ阳性主要见于ＤＬＣＬ，检出率为１５％，在非ＤＬＣＬ中ＰＣＲ－ＳＳＣＰ均为阴性（Ｐ＜０．０５）。表明国人ＮＨＬ，尤其在恶性程度很高的ＤＬＣＬ中存在ｐ５３基因突变，各型ＮＨＬ都有不同程度的突变型ｐ５３蛋白表达 The types and frequency of mutations in p53 exons 5 to 8 were studied by ABC and PCR-SSCP in 48 cases of NHL. The frequencies of p53 and p53 were detected by ABC and PCR-SSCP, and their relationship with pathological types and malignancy were also studied. Results: The positive rate of mutant p53 protein in NHL was 41.7%, which was 75% in diffuse large cell lymphoma (DLCL) and 35% in non-DLCL, the difference was significant (P <0. PCR-SSCP was negative in non-DLCL (P <0.05). The positive rate of PCRSSCP was mainly found in DLCL in p53-positive NHL. It is shown that there are p53 gene mutations in Chinese NHL, especially in the highly malignant DLCL, and all kinds of NHL have different degrees of mutant p53 protein expression