[Objective] To establish the simultaneous determination method for bergapten and isopimpinellin contents in FRUCTUS CNIDII.[Methods]Bergapten and isopimpinellin contents were detected by HPLC. Chromatographic column was Sino Chrom C18( 250 mm ×4. 6 mm,5 μm,Elite); mobile phase was methanol- 0. 1% acetic acid solution( 55∶ 45); flow rate was 1. 0 mL / min; column temperature was 25 ℃;and detection wavelength was 310 nm. [Results]Bergapten showed good linear relationship within the range of 0. 031 5- 0. 945 μg / mL,with A = 2 385C- 6. 666( r = 0. 999 9); and isopimpinellin had good linear relationship within the range of 0. 029 6- 0. 888 μg / mL,with A =1 711 C + 1. 203( r = 0. 999 9). The average recovery rates were 99. 2% and 99. 1%,respectively. And the relative standard deviations were0. 91% and 0. 67%( n = 6). The solution was stable within 8 h under normal temperature. [Conclusions] HPLC-DAD method was rapid,accurate,reliable and repeatable,and could be sued for the quality control of FRUCTUS CNIDII. [Objective] To establish the simultaneous determination method for bergapten and isopiine inine content in FRUCTUS CNIDII. [Methods] Bergapten and isopimpinellin contents were detected by HPLC. Chromatographic column was Sino Chrom C18 (250 mm × 4. 6 mm, 5 μm, Elite) ; mobile phase was methanol- 0.1% acetic acid solution (55:45); flow rate was 1. 0 mL / min; column temperature was 25 ° C .; and detection wavelength was 310 nm. [Results] Bergapten showed good linear relationship within the range of 0. 031 5- 0. 945 μg / mL with A = 2 385C- 6. 666 (r = 0. 999 9); and isopimpinellin had good linear relationship within the range of 0. 029 6- 0 . 888 μg / mL, with A = 1 711 C + 1. 203 (r = 0. 999 9). The average recovery rates were 99.2% and 99.1%, respectively. And the relative standard deviations were 0. 91 % Conclusions] HPLC-DAD method was rapid, accurate, reliable and repeatable, and could be sued for the quality (n = 6) control of FRUCTUS CNIDII.