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目的调查某大型补给船船舱内细菌气溶胶的浓度及构成,了解与细菌气溶胶影响相关的健康状况。方法采用安德森CA6型及CA2型撞击式空气采样仪,对舱室细菌气溶胶进行定量富集和粒径大小的分析,通过细菌学分析方法对富集的细菌进行种属鉴定,对艇员的健康状况进行量表调查。结果 (1)调查区域细菌气溶胶浓度为102~103CFU/m3。(2)60.6%~73.9%的舱室细菌气溶胶颗粒粒径大小为0.65~4.7μm。(3)空气中的细菌分布在15个菌属,由31个菌种构成。革兰阳性细菌占85.1%,其中革兰阳性球菌最多(41.8%),其余为芽孢杆菌属(24.3%)和无芽孢革兰阳性杆菌(19.0%)。革兰阴性杆菌占14.9%,其中肠杆菌科细菌5.4%,非发酵菌9.5%。结论该补给船舱室均处于相对较低的空气微生物污染水平,将粒径和条件致病菌一并作为评价因素,仍存在健康安全的隐患。
Objective To investigate the concentration and composition of bacterial aerosols in the tank of a large-scale supply ship and to understand the health status associated with the effects of bacterial aerosols. Methods Anderson CA6-type and CA2-type impact air samplers were used to quantitatively enrich and quantify the bacterial aerosols in the compartments. Species identification of enriched bacteria was carried out by bacteriological analysis, Status of the scale survey. Results (1) The bacterial aerosol concentration in the surveyed area was 102 ~ 103CFU / m3. (2) The size of 60.6% ~ 73.9% of the bacterial aerosol particles in the compartment is 0.65 ~ 4.7μm. (3) Bacteria in the air are distributed in 15 genera and consist of 31 species of bacteria. Gram-positive bacteria accounted for 85.1%, of which Gram-positive cocci was the most (41.8%), and the rest were Bacillus (24.3%) and non-Bacillus gram-positive bacilli (19.0%). Gram-negative bacilli accounted for 14.9%, of which Enterobacteriaceae 5.4% and non-fermentative bacteria 9.5%. CONCLUSION The tank of feed tank is at a relatively low level of airborne microbial contamination, and the combination of particle size and pathogenic bacteria as the evaluation factor still poses health and safety risks.