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本研究对６６例不明原因的原发不育及３４例继发不育男性病人的精液标本同时进行去透明带地鼠卵穿透试验（ＨＯＰ）、精子尾部低渗肿胀试验（ＨＯＳ）与ＤＮＡ荧光染色的有效精子计数（ＥＳＣ）的检测和统计分析。结果在１００例中ＨＯＳ和ＨＯＰ以及ＥＳＣ与ＨＯＰ的符合率分别达到７８％与７４％；ＨＯＳ与ＥＳＣ与ＨＯＰ三者的符合率亦达到６４％，表明ＨＯＳ、ＥＳＣ与ＨＯＰ一样也有反映精子受精能力的作用，并认为ＨＯＳ与ＥＳＣ的标准分别采用≥６０％与≥２０×１０￣６较为合适。此外，继发不育组的ＨＯＳ与ＨＯＰ符合率、ＥＳＣ与ＨＯＰ符合率以及ＨＯＳ与ＥＳＣ与ＨＯＰ三者符合率还明显高于原发不育组。
In this study, 66 cases of unexplained primary infertility and 34 cases of infertile male patients with semen samples were also tested for HOP, HOS and DNA Fluorescent staining of effective sperm count (ESC) detection and statistical analysis. Results The coincidence rates of HOS, HOP, ESC and HOP were 78% and 74% respectively in 100 cases. The coincidence rate of HOS, ESC and HOP was also 64%, indicating that HOS, ESC and HOP also reflect sperm fertilization The role and that HOS and ESC standards were ≥ 60% and ≥ 20 × 10 ~ 6 is more appropriate. In addition, the coincidence rate of HOS and HOP, the coincidence rate of ESC and HOP and the coincidence rate of HOS and ESC and HOP in the secondary infertility group were also significantly higher than those in the primary infertility group.