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为探讨多器官功能衰竭(MOF)的发病机理,应用饲菌复合失血性休克再灌注损伤的方法复制家兔MOF模型,依诊断标准实验组17只动物有12只发生MOF(70.6％)。用放射免疫分析法检测了血浆血管活性肠肽(VIP)的动态变化。MOF动物(n=12)实验前血浆VIP含量为20.17±4.45ng/L,实验后1.5h明显升高(43.67±0.35ng/L),超过实验前1倍,相差显著(P<0.05),尔后逐渐下降,72h降至最低值(5.47±2.68ng/L),只有实验前的1/4,相差显著(P<0.05),同对照组(n=6)相差亦非常显著(P<0.01)。结果表明,VIP作为一种重要的胃肠调节肽,对MOF的发生发展具有明显的病理生理意义。
To investigate the pathogenesis of multiple organ failure (MOF), rabbit MOF model was replicated by combined hemorrhagic shock and reperfusion injury in rabbits. According to the diagnostic criteria, MOF was found in 17 animals (70.6%) in 17 animals. The dynamic changes of plasma vasoactive intestinal peptide (VIP) were detected by radioimmunoassay. The plasma VIP level of MOF animals (n = 12) before experiment was 20.17 ± 4.45ng / L, significantly increased at 1.5h after the experiment (43.67 ± 0.35ng / L), exceeding the value of 1 time before experiment (P <0.05) (P <0.05), and the difference with the control group (n = 6) was also significant (P <0.01), then decreased gradually to the lowest level at 72h (5.47 ± 2.68ng / L) ). The results showed that VIP as an important gastrointestinal regulatory peptide has obvious pathophysiological significance on the occurrence and development of MOF.