论文部分内容阅读
采用多聚酶链反应结合ＨａｅⅢ、ＨｐａⅡ、ＡｌｕⅠ、ＴａｑⅠ四种限制性内切酶酶切及异源双链形成实验，分析了３０例急性淋巴细胞白血病（ＡＬＬ）患者发病及病程中的ＴＣＲγ、ＩｇＨ基因重排，探讨上述方法在完善微小残留病检测中的应用和临床意义。３０例ＡＬＬ患者，１６例呈ＴＣＲγ重排，６例呈ＩｇＨ基因重排，其中１０例进行了酶切与异源双链形成实验。结果表明，每例患者均有不同的Ｖ区参与重排，形成不同的异源双链，且在重排中常有Ｎ区插入，形成新的酶切位点与异源双链，从而产生新的基因标志。其独特的基因重排方式具高度特异性，有助于微小残留病的检测及部分演化克隆的识别。此外异源双链的形成亦有助于双等位基因重排与寡克隆的识别
Using polymerase chain reaction combined with four kinds of restriction endonuclease digestion and heteroduplex formation experiments including HaeIII, HpaII, AluI, and TaqI, the TCRγ and IgH genes in the pathogenesis and course of disease in 30 patients with acute lymphoblastic leukemia (ALL) were analyzed. Rearrangement to explore the application and clinical significance of the above methods in the detection of minimal residual disease. In 30 patients with ALL, 16 patients had TCRγ rearrangement, and 6 patients showed IgH gene rearrangement, of which 10 patients underwent enzyme digestion and heteroduplex formation experiments. The results showed that each patient had different V regions involved in rearrangement, forming different heteroduplexes, and often N regions were inserted in the rearrangements to form new enzyme cleavage sites and heteroduplexes, resulting in new Genetic marker. Its unique gene rearrangement method is highly specific, and it is helpful for the detection of minimal residual disease and the identification of partially evolved clones. In addition, the formation of heteroduplexes also contributes to the identification of biallel rearrangements and oligoclonal clones.