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目的观察慢性髓细胞白血病（ＣＭＬ）病情恶化与降钙素（ＣＴ）基因高甲基化程度之间的关系。方法用内切酶酶切和ＰＣＲ技术研究４５例ＣＭＬ患者（其中慢性期３３例，加速期２例，急变期１０例）的ＣＴ基因高甲基化程度。ＣＴ基因未高甲基化，则能被相应内切酶切断，无法扩增出特异ＤＮＡ片断，否则，能扩增出特异ＤＮＡ片断，据此推断ＤＮＡ的甲基化程度。结果３例慢性期，２例加速期及８例急变期患者呈现降钙素基因高甲基化；急变期组（包括加速期）甲基化率明显高于慢性期组，两组有显著性差别（Ｐ＜０．０１）；１例在慢性期呈现ＣＴ基因高甲基化者于１０个月后发生急粒变。结论该方法有助于监测ＣＭＬ的恶化
Objective To observe the relationship between the deterioration of chronic myeloid leukemia (CML) and hypermethylation of calcitonin (CT) gene. Methods The degree of hypermethylation of CT gene was studied in 45 CML patients (33 in the chronic phase, 2 in the accelerated phase, and 10 in the blast phase) by restriction endonuclease digestion and PCR. If the CT gene is not hypermethylated, it can be cleaved by the corresponding endonuclease, and no specific DNA fragment can be amplified. Otherwise, the specific DNA fragment can be amplified and the degree of DNA methylation can be deduced. Results The hypermethylation of calcitonin gene was found in 3 patients with chronic phase, 2 patients with accelerated phase and 8 patients with blast crisis. The methylation rate in the blast crisis phase (including the acceleration phase) was significantly higher than that in the chronic phase. There was a significant difference between the two groups ( P<0.01); 1 patient presented with hypermethylation of CT gene in the chronic phase had acute granuloma after 10 months. Conclusion This method helps to monitor the deterioration of CML