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目的:观察6周耐力训练及补充多糖提取物对大鼠血清IL-1β和外周血单核细胞(PBMC)培养后上清液IL-1β含量、下丘脑室旁核IL-1R表达和血清皮质酮、睾酮含量的影响。方法:6周龄雄性Wistar大鼠40只随机分为安静对照组、耐力训练组、训练+黄芪多糖组、训练+牛膝多糖组4组,每组10只。安静对照组不运动,其他3组进行6周递增负荷游泳训练,训练时间由40 min/d逐渐增加到160 min/d。训练+黄芪多糖组每天给予黄芪多糖提取物灌胃(2 g.kg-1),训练+牛膝多糖组给予牛膝多糖提取物灌胃(1 g.kg-1),安静对照组和耐力训练组给予等量双蒸水。6周后取材测试相关指标。结果:6周训练后,耐力训练组大鼠血清IL-1β含量、PBMC培养后上清液IL-1β含量、大鼠下丘脑室旁核IL-1R表达、血清皮质酮含量均显著高于安静对照组(P<0.05或P<0.01),血清睾酮含量显著低于安静对照组(P<0.05)。训练同时补充牛膝多糖组大鼠血清IL-1β、下丘脑室旁核IL-1R表达显著低于耐力训练组(P<0.05或P<0.01),且与安静对照组水平接近;训练同时补充牛膝多糖或黄芪多糖组PBMC生成IL-1β能力低于耐力训练组、高于安静对照组,但差异均无统计学意义,训练同时补充牛膝多糖组血清皮质酮显著低于耐力训练组(P<0.01)。训练同时补充牛膝多糖或黄芪多糖组大鼠血清睾酮高于耐力训练组(分别高54.3%和54.4%)、低于安静对照组(分别低21.4%和21.2%)。结论:耐力训练引起细胞免疫功能下降可能与血清及PBMC中IL-1β升高和下丘脑室旁核IL-1R表达增加并激活HPA轴有关,补充牛膝多糖、黄芪多糖有保护作用。
OBJECTIVE: To observe the changes of IL-1β, IL-1R in hypothalamic paraventricular nucleus and serum cortex of rat serum after IL-1β and PBMC cultured for 6 weeks. Ketone, testosterone content. Methods: Forty male Wistar rats, 6 weeks old, were randomly divided into 4 groups (quiet control group, endurance training group, training + astragalus polysaccharide group, training + Achyranthes bidentata polysaccharide group, 10 rats in each group). Quiescent control group did not exercise, the other three groups of 6-week incremental load swimming training, training time gradually increased from 40 min / d to 160 min / d. Astragalus polysaccharide group was given gavage (2 g.kg-1) daily for training + Astragalus polysaccharide group, gavage (1 g.kg-1) The training group was given the same amount of double distilled water. 6 weeks after drawing test related indicators. Results: After training for 6 weeks, the levels of IL-1β in serum, IL-1β in supernatant of PBMC, IL-1R in paraventricular nucleus of hypothalamus, and corticosterone in serum of endurance training group were significantly higher than those in resting The level of serum testosterone in the control group (P <0.05 or P <0.01) was significantly lower than that in the quiet control group (P <0.05). Compared with the endurance training group (P <0.05 or P <0.01), the expression of IL-1β in the serum and the paraventricular nucleus of the hypothalamus of training group were significantly higher than those in the control group Achyranthes polysaccharide or Astragalus polysaccharide group PBMC IL-1β production ability lower than the endurance training group, higher than the quiet control group, but the difference was not statistically significant, while training supplemented with Achyranthes polysaccharide serum corticosterone was significantly lower than the endurance training group P <0.01). Serum testosterone was significantly higher in training group than in training group (54.3% and 54.4%, respectively) and lower than that in quiet control group (21.4% and 21.2% respectively). CONCLUSION: The decrease of cellular immune function induced by endurance training may be related to the increase of IL-1β in serum and PBMC, the increase of IL-1R expression in paraventricular nucleus of hypothalamus and the activation of HPA axis, and the supplementation of Achyranthes bidentata polysaccharide and Astragalus polysaccharide.