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目的：初探炎性刺激对人多核白细胞（ＰＭＮ）和脐静脉内皮细胞（ＨＵＶＥＣ）的血小板内皮细胞粘附分子－１（ＰＥＣＡＭ－１）表达的影响和ＰＥＣＡＭ－１的可能作用。方法：使用流式细胞仪和免疫组化法，观察脂多糖（ＬＰＳ）、白介素（ＩＬ）－１β和肿瘤坏死因子（ＴＮＦ）－ａ对ＰＭＮ和ＨＵＶＥＣ ＰＥＣＡＭ－１表达的变化。结果：ＬＰＳ、１Ｌ－１β和ＴＮＦ－ａ可引起ＰＭＮ表达ＰＥＣＡＭ－１蛋白减少，而对ＨＵＶＥＣ ＰＥＣＡＭ－１的表达无显著影响，但组化显著在ＨＵ－ＶＥＣ连接部的ＰＥＣＡＭ－１染色变淡。结论：上述炎性刺激物不仅可引起ＰＭＮ表达ＰＥＣＡＭ－１减少、激活ＰＭＮ，而且可改变内皮细胞ＰＥＣＡＭ－１的分布，因此ＰＥＣＡＭ－１在炎症中可能起重要作用。
Objective: To investigate the effect of inflammatory stimuli on the expression of platelet adhesion molecule-1 (PECAM-1) and the possible role of PECAM-1 in human polymorphonuclear leukocytes (PMN) and human umbilical vein endothelial cells (HUVEC). Methods: The changes of PECAM-1 expression in PMN and HUVEC were observed by flow cytometry and immunohistochemistry in the presence of lipopolysaccharide (LPS), interleukin (IL) -1β and tumor necrosis factor (TNF) -a. RESULTS: LPS, 1L-1β and TNF-α induced a decrease of PMN-expressing PECAM-1 protein but no significant effect on the expression of PECAM-1 in HUVEC. However, the staining of PECAM-1 in HU-VEC junction decreased significantly . Conclusion: The above inflammatory stimuli not only cause PMN to decrease PECAM-1, activate PMN, but also change the distribution of PECAM-1 in endothelial cells. Therefore, PECAM-1 may play an important role in inflammation.