通过化学方法和电融合法对白芝(Ganoderma applanatum,GA)和赤芝(Ganoderma lucidum,GL)两种大型真菌原生质体融合条件进行了研究,实验得出:化学方法对灵芝菌原生质体融合的最佳条件:PEG浓度30%、CaCl20.02mol/L、融合时间30min、温度30℃,融合率可达0.4%;电融合法对灵芝菌原生质体融合的最佳条件:交变电流频率2MHz、电压40V、直流脉冲脉幅10kV/cm、脉宽25μs、直流电压为500V,融合率可达40%。 The fusion conditions of Ganoderma lucidum (Ganoderma lucidum, GA) and Ganoderma lucidum (GL) were studied by means of chemical and electrofusion methods. The results showed that the best fusion method of Ganoderma lucidum protoplast The optimal conditions for the fusion of Ganoderma lucidum protoplasts by electrofusion were as follows: the frequency of alternating current was 2MHz, the voltage was 40V, the optimal conditions were as follows: PEG concentration 30%, CaCl20.02mol / L, fusion time 30min, temperature 30 ℃, , DC pulse pulse amplitude 10kV / cm, pulse width 25μs, DC voltage 500V, the fusion rate of up to 40%.