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以堆肥作为3株功能芽胞细菌液体菌剂的载体,通过优化载体含水量、温度和接种浓度等关键影响因子,以不同时间载体中有效活菌数的变化为指标,探讨堆肥代替草炭作为功能微生物载体的可行性和最适条件。研究结果表明,载体C(鸡粪)、P(猪粪)、M(1∶1鸡粪猪粪)和TP(猪粪+草炭)在72 h内的有效活菌数均显著低于草炭;混合载体TC2(50%草炭+50%鸡粪)和TM1(25%草炭+75%1∶1鸡粪猪粪)的有效活菌数随着时间的延长而增加,其中72 h时TC2的有效活菌数达到11.40×109 cfu.g-1,TM1的有效活菌数达到2.64×109 cfu.g-1,均与草炭无显著差异,因此适宜代替草炭作为功能微生物的载体。采用单因素实验,载体TC2和TM1的最优化影响因子为含水量30%、吸附温度30℃、菌液接种浓度为108cfu.mL-1。
Compost was used as the carrier of the three bacterial spores bacteriocin. Through the optimization of the key influencing factors such as water content, temperature and inoculum concentration, the effective viable count in the vector at different times was taken as an index to discuss the compost as the substitute for peat Carrier’s feasibility and optimum conditions. The results showed that the viable count of C (chicken manure), P (pig manure), M (1: 1 chicken manure pig manure) and TP (pig manure + peat) within 72 h were significantly lower than that of peat. The effective viable count of mixed carrier TC2 (50% peat + 50% chicken manure) and TM1 (25% peat + 75% 1: 1 chicken manure) increased with time, of which TC2 was effective at 72 h The number of viable cells reached 11.40 × 109 cfu.g-1, and the viable count of TM1 reached 2.64 × 109 cfu.g-1, which showed no significant difference from that of peat. Therefore, it was suitable to replace peat as a carrier of functional microorganisms. Using single-factor experiments, the optimal factors of TC2 and TM1 were 30% water content, 30 ℃ adsorption temperature and 108cfu.mL-1 concentration.