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目的探讨肿瘤细胞转移潜能与７２０００ＩＶ型胶原酶的关系。方法采用明胶酶谱分析法，检测两组５种不同转移潜能的人癌细胞系，分泌７２０００ＩＶ型胶原酶的情况，以及人正常纤维母细胞在不同癌细胞的作用下，分泌的７２０００ＩＶ型胶原酶活性的改变。结果高转移潜能的癌细胞（ＰＧ、ＷＭ４５１和ＷＭ９８３ａ）分泌７２０００ＩＶ型胶原酶的量明显高于低转移潜能的癌细胞（ＰＡａ和ＷＭ３５）；在受ＰＧ和ＷＭ４５１细胞作用的纤维母细胞的条件培养液中，活化型７２０００ＩＶ型胶原酶的产量明显高于其他癌细胞作用的纤维母细胞。结论７２０００ＩＶ型胶原酶的表达和分泌与肿瘤细胞的转移潜能密切相关；高转移潜能的癌细胞（ＰＧ）和转移瘤细胞（ＷＭ４５１）可能通过分泌某些可溶性介质，刺激纤维母细胞，促进酶原型７２０００ＩＶ型胶原酶的活化，从而更有效地降解ＩＶ型胶原，达到转移的目的。
Objective To investigate the relationship between the metastatic potential of tumor cells and 72000 type IV collagenase. METHODS: Gelatin zymography was used to detect 5 cancer cell lines with different metastatic potentials, and the secretion of 72000 type IV collagenase and the secretion of 72000 type IV collagenase from human normal fibroblasts under the action of different cancer cells. Change in activity. Results The metastatic potential of cancer cells (PG, WM451 and WM983a) secreted 72000 type IV collagenase significantly higher than that of low metastatic potential cancer cells (PAa and WM35); conditional culture of fibroblasts affected by PG and WM451 cells In solution, the production of activated 72000 type IV collagenase was significantly higher than that of other fibroblasts that acted on other cancer cells. Conclusion The expression and secretion of type 7 collagenase IV are closely related to the metastatic potential of tumor cells; high metastatic potential cancer cells (PG) and metastatic tumor cells (WM451) may stimulate fibroblasts by promoting the secretion of certain soluble mediators and promote the enzyme prototype. The activation of 72000 type IV collagenase can effectively degrade type IV collagen and achieve the purpose of metastasis.