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人食管癌细胞株 EC109以显微分光光度计测定癌细胞核 DNA 含量,以放射自显影测定细胞核摄取~3H-TdR 和扫描电镜检查细胞表面的超微结构.大多数 EC109瘤细胞间期细胞核的 DNA 含量增加,主要干系细胞 DNA 含量为2 C,属 G_1期,也可见低于四倍体(3 C),高于四倍体(>4 C).甚至多倍体(>8 C)的瘤细胞.~3H-TdR 加入培养基10分钟后,许多瘤细胞核有银粒出现(S 期细胞).大量细胞进入细胞周期反映其恶性程度高.扫描电镜可见 EC109在细胞周期不同阶段其细胞大小形状和微绒毛也不同.本文结果说明用细胞核 DAN 含量测定,~3H-TdR 掺入和细胞表面超微结构可测定肿瘤周期.也可用这些方法判定瘤细胞的恶性程度.
Human esophageal cancer cell line EC109 was used to measure the nuclear DNA content of cancer cells by microscopic spectrophotometer. The nuclear uptake of ~3H-TdR was determined by autoradiography and the ultrastructure of the cell surface was examined by scanning electron microscopy. The majority of EC109 tumor cell interphase nuclear DNA. The content of DNA in the main stem cells is 2 C, which belongs to G 1 phase. It also shows lower tetraploid (3 C), higher than tetraploid (>4 C), and even polyploid (>8 C) tumors. After cells were added with ~3H-TdR for 10 minutes, many tumor cells showed silver granules (S phase cells). A large number of cells entered the cell cycle to reflect their high degree of malignancy. Scanning electron microscopy showed that EC109 cell size and shape at different stages of the cell cycle And microvilli are also different. This article shows that using the nucleus DAN content, ~3H-TdR incorporation and cell surface ultrastructure can determine the tumor cycle. These methods can also be used to determine the degree of malignancy of tumor cells.