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目的：探讨ＤＮＡ含量、核形态参数对预测上皮性卵巢癌患者预后的价值。方法：应用计算机图像分析系统测定原发性上皮性卵巢癌３２例的细胞核ＤＮＡ含量、倍体水平、面积、周长及形状因子５个参数。结果：卵巢癌临床晚期及转移病例的ＤＮＡ含量明显增高，与早期及无转移组相比，差异均有显著性（Ｐ＜０．０２５）。６３％为异倍体肿瘤，３７％为二倍体肿瘤。组织学Ⅰ、Ⅱ、Ⅲ级的异倍体率分别为３８％、５７％、９０％，呈递增趋势。Ⅰ级与Ⅲ级相比，差异有显著性（Ｐ＜０．０５）。腹水阳性组及转移组的异倍体率明显增加，分别为７３％及７６％。ＰＣＮＡ阳性组及阴性组的核形状因子差异有显著性（Ｐ＜０．０５）。核面积和周长在诸预后因素间差异无显著性（Ｐ＞０．０５）。结论：细胞核ＤＮＡ含量和倍体水平是影响卵巢癌患者预后的重要因素；核形状因子亦可作为评估肿瘤生物学行为的一项指标。
Objective: To investigate the value of DNA content and nuclear morphological parameters in predicting the prognosis of patients with epithelial ovarian cancer. Methods: Computer image analysis system was used to determine the nuclear DNA content, ploidy level, area, perimeter and shape factor of 32 patients with primary epithelial ovarian cancer. RESULTS: The DNA content of advanced ovarian cancer cases and metastatic cases was significantly higher than that of the early and non-metastasis groups (P<0.025). 63% were aneuploid tumors and 37% were diploid tumors. The aneuploidy rates of grades I, II, and III of histology were 38%, 57%, and 90%, respectively, showing an increasing trend. The difference between grade I and grade III was significant (P < 0.05). Ascites positive group and metastasis group significantly increased the aneuploidy rate of 73% and 76%. There was a significant difference in the nuclear shape factor between PCNA positive group and negative group (P<0.05). Nuclear area and perimeter had no significant difference among the prognostic factors (P>0.05). Conclusion: The nuclear DNA content and ploidy level are important factors affecting the prognosis of ovarian cancer patients; nuclear shape factor can also be used as an index to evaluate the biological behavior of tumors.