运用双层垂直板聚丙烯酰胺凝胶电泳方法,对杂交水稻苗期的酯酶、过氧化物酶、细胞色素氧化酶、多酚氧化酶、谷草转氨酶、β-淀粉酶、6-磷酸葡萄糖脱氢酶同工酶、苹果酸脱氢酶同工酶进行电泳分析,探索水稻苗期同工酶与杂种优势的关系。结果表明,不同的同工酶与杂种优势相关性大小不同,其中酯酶、过氧化物酶、细胞色素氧化酶、多酚氧化酶、谷草转氨酶同工酶与杂种优势都有一定的相关性,以酯酶同工酶的相关性为最大。本文尝试用差异指数法来预测水稻的杂种优势。 Double-layer vertical plate polyacrylamide gel electrophoresis was used to detect the contents of esterase, peroxidase, cytochrome oxidase, polyphenol oxidase, aspartate aminotransferase, β-amylase, Hydrogenase isozymes and malate dehydrogenase isozymes were analyzed by electrophoresis to explore the relationship between rice seedling isozymes and heterosis. The results showed that the correlations of different isozymes and heterosis were different. Esterase, peroxidase, cytochrome oxidase, polyphenol oxidase and aspartate aminotransferase isozymes had some correlation with heterosis, The esterase isoenzyme is the most relevant. This article attempts to use the difference index method to predict the heterosis of rice.