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目的 探讨直接识别和间接识别在异种移植中的作用。方法 分别以中国内江猪和人的外周血淋巴细胞做为刺激细胞和效应细胞,进行异种单向混合淋巴细胞反应,并与同种异体和自体混合淋巴细胞反应作对照,采用3H-TdR检测混合反应的增殖性。结果 在三种混合方式中,同种异体混合淋巴细胞反应的增殖最强,其次为异种混合,自体混合的增殖最低[采用改良Terasaki血清法对人和猪进行HLA-A、B、DR、DQ 配型,人H1 为A 9,33+ 34;B27,16(40);DR4,8;DQ 1,- 。人H2为A 29(10),23(9);B22,27(7);DR12,9(52);DQ 2,3。]。猪配型不成功。结论 从单向混合淋巴细胞反应的结果提示主要组织相容复合物(MHC)的适配性在异种移植中仍起作用;异种移植中,MHC配型可能也值得考虑,但现行血清法配型不适合于猪的配型
Objective To explore the role of direct recognition and indirect identification in xenotransplantation. Methods The peripheral blood lymphocytes of Neijiang pig and human in China were used as stimulator cells and effector cells, respectively, for heterogeneous unidirectional mixed lymphocyte reaction. Compared with allogeneic and autologous mixed lymphocyte reaction, 3H-TdR assay Reactivity of the reaction. Results Among the three mixed modes, allogeneic mixed lymphocyte reaction proliferated most strongly, followed by xeno-mixed, with the lowest self-mixed proliferation [HLA-A, B, DR, DQ Configuration, human H1 is A 9,33 + 34; B27,16 (40); DR4,8; DQ 1, -. Human H2 is A 29 (10), 23 (9); B22,27 (7); DR12,9 (52); DQ 2,3. ]. Pig matching unsuccessful. Conclusions The results from unidirectional mixed lymphocyte reaction suggest that the adaptability of major histocompatibility complex (MHC) still plays a role in xenotransplantation; MHC mapping may also be worth considering in xenotransplantation, Not suitable for pig type