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近来在成人和胚胎干细胞培养方面的成绩,增加了这些细胞应用于神经退化性疾病和癌症等疾病治疗的希望。 用饲养细胞培养干细胞或用细胞因子处理细胞对于维持干细胞的未分化状态和保持它们的多潜能性是必要的。特别是,细胞因子白血病抑制因子(LIF)已经用来在缺乏饲养细胞的情况下培养小鼠胚胎干细胞(ES)。由于未知的原因.LIF不能对大鼠和人干细胞引起同样的效应。德国Rose-John S的这篇文章对培养干细胞方面已知的和相关的问题进行了概括,提出了有助于克服这些困难的实验战略。
Recent achievements in adult and embryonic stem cell culture have increased the hope that these cells should be used in the treatment of diseases such as neurodegenerative diseases and cancers. Incubation of stem cells with feeder cells or treatment of cells with cytokines is necessary to maintain the undifferentiated state of stem cells and maintain their pluripotency. In particular, cytokine leukemia inhibitory factor (LIF) has been used to culture mouse embryonic stem cells (ES) in the absence of feeder cells. For unknown reasons, LIF can not cause the same effect on rat and human stem cells. This article by Rose-John S in Germany summarizes the known and relevant problems in stem cell culture and suggests experimental strategies that will help overcome these difficulties.