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目的:检测胃癌、结肠癌及其相应正常组织中端粒酶活性,探讨其在胃癌、结肠癌发生中的作川以及与临床病理特征之间的关系。方法:采用PCR-TRAP法检测92例胃癌、结肠癌和61例正常组织的端粒酶活性,并州端粒酶活性与临床病理特征关系进行分析。结果:胃癌和结肠癌中端粒酶活性阳性率为84.9％,而正常组织为9.83％,胃癌和结肠癌组织端粒酶活性显著高于正常组织,P<0.01;胃癌、结肠癌组织中端粒酶活性在不同分化程度及淋巴转移之间未发现显著差异,P>0.05。结论:端粒酶在胃癌、结肠癌的发生中起重要作用;端粒酶在胃癌、结肠癌组织中高表达,而在正常胃肠组织低表达的特性,使其有望成为一种较为理想的胃肠道肿瘤诊断的标志物。
OBJECTIVE: To detect the telomerase activity in gastric cancer, colon cancer and its corresponding normal tissues and to explore its relationship with clinicopathological features in gastric cancer and colon cancer. Methods: The telomerase activity of 92 cases of gastric cancer, colon cancer and 61 normal tissues was detected by PCR-TRAP, and the relationship between telomerase activity and clinicopathological features was analyzed. Results: The positive rate of telomerase activity in gastric cancer and colon cancer was 84.9%, while it was 9.83% in normal tissues. The telomerase activity in gastric cancer and colon cancer tissues was significantly higher than that in normal tissues (P <0.01). In gastric cancer and colon cancer Granzyme activity in different degrees of differentiation and lymph node metastasis was not significantly different, P> 0.05. Conclusion: Telomerase plays an important role in the carcinogenesis of gastric cancer and colon cancer. Telomerase is highly expressed in gastric cancer and colon cancer tissues, but low in normal gastrointestinal tissues, making it an ideal gastric A marker of intestinal cancer diagnosis.