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目的探讨腺病毒36型(Ad36)感染对维吾尔族肥胖患者颗粒蛋白前体(progranulin)表达的调节作用。方法根据肥胖诊断标准将维吾尔族人群样本分为肥胖组(115)与非肥胖组(117),收集血清标本232份,脂肪标本102份。血清中和反应法检测样本血清中的Ad36抗体。采用实时荧光定量PCR(real time quantitative-PCR)方法检测研究对象大网膜和皮下的脂肪组织progranulin的mRNA表达水平,利用酶联免疫吸附实验(ELISA)测定血清progranulin的蛋白表达水平。免疫组化方法检测脂肪组织巨噬细胞CD68蛋白表达,了解巨噬细胞浸润情况。结果 1肥胖患者Ad36感染率(54/115,47.0%)明显高于非肥胖者(38/117,32.5%),差异有统计学意义(P<0.05)。2Ad36感染的肥胖组血清progranulin的蛋白表达水平(408.45±156.92)显著高于非肥胖组(326.11±158.60),差异有统计学意义(P<0.05);两组的腹部大网膜、皮下脂肪组织progranulin mRNA表达水平差异无统计学意义(P均>0.05)。3Ad36感染的肥胖组巨噬细胞浸润(14 730.16±2 227.39)明显高于非肥胖组(10 786.50±2 772.80),差异有统计学意义(P<0.05)。结论 Ad36感染可能与维吾尔族肥胖发生有关,其机制可能为通过调节血清progranulin的蛋白表达水平而实现。
Objective To investigate the regulatory effect of adenovirus type 36 (Ad36) infection on the expression of progranulin in Uighur obese patients. Methods According to obesity diagnostic criteria, Uyghur population was divided into obesity group (115) and non-obesity group (117). 232 serum samples and 102 fat samples were collected. Serum neutralization assay samples for serum Ad36 antibody. Real-time quantitative PCR (Real-time quantitative-PCR) was used to detect the mRNA expression level of progranulin in omentum and subcutaneous adipose tissue. The protein expression of progranulin was detected by enzyme linked immunosorbent assay (ELISA). Immunohistochemical method was used to detect the expression of CD68 protein in adipose tissue macrophages to understand the infiltration of macrophages. Results The prevalence of Ad36 infection in obese patients (54 / 115,47.0%) was significantly higher than that in non-obese patients (38 / 117,32.5%). The difference was statistically significant (P <0.05). The protein expression level of progranulin in obese group (408.45 ± 156.92) was significantly higher than that in non-obese group (326.11 ± 158.60) (P <0.05). The abdominal omental, subcutaneous adipose tissue Progranulin mRNA expression levels were not statistically different (all P> 0.05). The number of macrophage infiltration in 3Ad36-infected obese group (14 730.16 ± 2 227.39) was significantly higher than that in non-obese group (10 786.50 ± 2 772.80), the difference was statistically significant (P <0.05). Conclusion Ad36 infection may be related to the occurrence of obesity in Uighur. The possible mechanism may be through regulating the protein expression of progranulin in serum.