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目的了解轮状病毒在福州地区腹泻儿童中的流行情况。方法收集2009—2014年5岁以下腹泻住院儿童粪便标本,用ELISA法检测轮状病毒抗原,RT-PCR法确定基因型别。对G9轮状病毒阳性标本的VP7基因全长测序及进化分析。结果福州地区腹泻儿童轮状病毒高峰期在10~12月,呈单峰流行态势。G9轮状病毒在2011年后成为福州地区优势流行型别,毒株VP7基因核苷酸序列相似性92.5%~100%,毒株间有较高的同源性,进化分析都属于G9第3亚型。结论 G9轮状病毒在福州地区流行强度逐渐加强,目前已成为优势流行型别。毒株同源性高,属G9型3亚型。
Objective To understand the prevalence of rotavirus in children with diarrhea in Fuzhou. Methods Stool specimens from hospitalized children with diarrhea under 5 years old from 2009 to 2014 were collected. The rotavirus antigen was detected by ELISA and the genotypes were determined by RT-PCR. Full - length sequencing and evolution analysis of VP7 gene of G9 rotavirus positive specimen. Results The peak period of rotavirus in children with diarrhea in Fuzhou was from January to December, showing a single peak epidemic trend. G9 rotavirus became the dominant epidemic type in Fuzhou after 2011. The nucleotide sequence similarity of the VP7 gene of the strain was 92.5% -100%, and the homology was high among the strains. The evolutionary analysis belonged to G9 / 3 Subtype. Conclusion The epidemic intensity of G9 rotavirus in Fuzhou area has been gradually strengthened and has now become the dominant epidemic type. Strain homology is high, is a G9 type 3 subtype.