[Objective]The paper was to explore effects of Tomato aspermy virus(TAV)-free on chlorophyll content and nitrogen metabolism of Chrysanthemum morifolium Ramat. ‘Huaibai’,in order to provide a theoretical basis for field popularization and application of TAV-free‘Huai bai’plantlets. [Method]With previously acquired TAV-free test-tube plantlets of‘Huaibai’as the experimental materials and its ordinary test-tube plantlets as the control(CK),chlorophyll content and some indexes related to nitrogen metabolism in leaves were determined during subculture.[Result]During subculture,the contents of chlorophyll a and chlorophyll b in leaves of CK and TAV-free‘Huaibai’test-tube plantlets increased with the subculture days. Both realized the largest increase at the 22~(th) day of subculture,and their increase amplitudes were 5. 24% and 41. 69%,respectively. Compared with CK,the chlorophyll a and chlorophyll b contents of TAV-free test-tube plantlets increased at the same time point of subculture,with significant difference(P < 0. 05) or extremely significant difference(P<0. 01). Furthermore,the nitrate reductase(NR) activity,free proline and soluble protein contents of TAV-free‘Huaibai’were all significantly higher than those of CK,and the highest increase amplitudes were 27. 99%(subculture for 22 d),28. 10%(subculture for 22 d) and 5. 89%(subculture for 38 d),respectively.[Conclusion]TAV-free could effectively improve chlorophyll content and nitrogen metabolism level of‘Huaibai’,and was conducive to its growth and development. [Objective] The paper was to explore effects of Tomato aspermy virus (TAV) -free on chlorophyll content and nitrogen metabolism of Chrysanthemum morifolium Ramat. ’Huaibai’, in order to provide a theoretical basis for field popularization and application of TAV-free ’ [Method] With previously acquired TAV-free test-tube plantlets of’Huaibai’as the experimental materials and its ordinary test-tube plantlets as the control (CK), chlorophyll content and some indexes related to nitrogen metabolism in [Result] During subculture, the contents of chlorophyll a and chlorophyll b in leaves of CK and TAV-free’Huaibai’test-tube plantlets increased with the subculture days. Both realized the largest increase at the 22 ~ (th) day of subculture, and their increase amplitudes were 5. 24% and 41. 69%, respectively. Compared with CK, the chlorophyll a and chlorophyll b contents of TAV-free test-tube plantlets increased at the same time point of subcu In addition, the nitrate reductase (NR) activity, free proline and soluble protein contents of TAV-free’Huaibai’were all significantly (P <0.05) or extremely significant difference higher than those of CK, and the highest increase amplitudes were 27. 99% (subculture for 22 days), 28.10% (subculture for 22 days) and 5.89% (subculture for 38 days) TAV-free could effectively be able to improve chlorophyll content and nitrogen metabolism level of ’Huaibai’, and was conducive to its growth and development.