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目的:探讨乙型肝炎病毒X蛋白(hepatitis BvirusX protein,HBVX)对阿霉素诱导的肝癌细胞凋亡及p53、PTEN表达的影响。方法:用阿霉素(2.5g/mL)分别处理HepG及稳定表达GFP、GFP-HBVX融合蛋白的细胞系HepG/GFP、22HepG/GFP-HBVX,处理后不同时间在显微镜下观察细胞形态变化,流式细胞术检测细胞凋亡。结果:流式细胞术检测显2示阿霉素处理后36h,HepG/GFP-HBVX细胞凋亡率为3.94%,明显低于HepG(59.03%)、HepG/GFP细胞(61.38%)222(P<0.05),而与未处理对照组细胞(2.12%、2.78%、2.55%)无明显差别(P>0.05)。结论:HBVX蛋白能够抑制阿霉素诱导的细胞凋亡。
Objective: To investigate the effects of hepatitis B virus X protein (HBVX) on doxorubicin-induced hepatocellular carcinoma cell apoptosis and the expression of p53 and PTEN. Methods: The HepG cells and HepG / GFP and GFP-HBVX fusion cell lines stably expressing HepG and GFP-HBVX fusion protein were treated with doxorubicin (2.5g / mL) respectively. Cell morphology was observed under microscope at different times after treatment. Flow cytometry was used to detect apoptosis. Results: The apoptotic rate of HepG / GFP-HBVX cells was 3.94% at 36 h after Doxorubicin treatment, which was significantly lower than that of HepG (59.03%) and HepG / GFP cells (61.38%) 222 <0.05), while there was no significant difference between untreated control cells (2.12%, 2.78%, 2.55%) (P> 0.05). Conclusion: HBVX protein can inhibit doxorubicin - induced apoptosis.