中东杨插条苗经水分胁迫处理后,在扫描电镜下观察了气孔开度的变化,并用扫描电镜结合X-射线能谱显微分析技术,分析了保卫细胞中的离子含量变化。结果表明,随着水分胁迫强度的增大,气孔开度变小,气孔趋于关闭。伴随着气孔关闭,保卫细胞中最明显的变化就是K+、Cl-含量的下降和Ca2+含量的上升。这个结果表明K+、Ca2+等离子参与了气孔运动,K+、Ca2+的移动在保卫细胞膨压运动及气孔开闭现象中起着十分重要的作用。 After the seedlings of Populus deltoides were treated by water stress, the changes of stomatal opening were observed under the scanning electron microscope. The changes of ion content in guard cells were analyzed by scanning electron microscopy and X-ray energy dispersive spectroscopy. The results showed that the stomatal opening became smaller and the stomata tended to close with the increase of water stress intensity. With the stomatal closure, the most obvious changes in guard cells are the decrease of K + and Cl- content and the increase of Ca2 + content. This result indicates that K + and Ca2 + ions are involved in stomatal movement, and the movement of K + and Ca2 + plays an important role in guarding the turgor movement and stomatal opening and closing.