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目的 :建立测定大鼠血浆样品中川芎哚浓度的气相色谱 -质谱 (GC -MS)定量分析方法。方法 :血浆样品经乙醚 -异丙醇 (10∶1)提取、硅烷化处理后 ,选择GC -MS选择性离子和辅助定量离子监测。结果 :本法线性范围为 2～ 6 0ng·mL-1,灵敏度为 1ng·mL-1,回收率达 82 % ,日内日间RSD均小于 9 0 %。结论 :本法为川芎哚临床药代动力学研究提供了准确、可靠的方法。
Objective: To establish a gas chromatography-mass spectrometry (GC-MS) quantitative method for the determination of Chuanxiongodole in rat plasma samples. Methods: The plasma samples were extracted with ether-isopropanol (10: 1) and silanized. GC-MS selective ion and assisted quantitative ion monitoring were selected. Results: The linear range of this method was 2 ~ 60 ng · mL-1, the sensitivity was 1 ng · mL-1 and the recovery rate was 82%. The intra-day and inter-day RSD were less than 90%. Conclusion: This method provides an accurate and reliable method for the study of pharmacokinetics of ligusticum.