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[目的]探讨胃癌组织细胞内氯离子通道蛋白1 (CLIC1 )蛋白表达与胃癌侵袭转移的关系.[方法]检测 94例胃癌和相应癌旁组织中CLIC1 mRNA及蛋白的表达并分析其差异性和胃癌组织 CLIC1 蛋白表达与临床病例特征的相关性.采用多因素Cox比例模型分析胃癌预后不良的影响因素.[结果]CLIC1蛋白在胃癌组织中的阳性表达率为 86.17%(81／94),显著高于癌旁组织的 24.47%(23／94)(P＜0.01).胃癌组织CLIC1 蛋白表达与患者年龄、性别、远处转移和肿瘤部位无关,而与组织分化程度、浸润深度、淋巴结转移及TNM分期有关(P＜0.05).CLIC1mRNA在胃癌组织中的表达水平较癌旁正常组织明显升高(P＜0.05).CLIC1 低表达的胃癌患者的中位生存时间为 29 个月,显著高于高表达患者的 25 个月(P＜0.01),且低表达者的五年生存率显著长于高表达者(P＜0.05).浸润深度、淋巴结转移、部位(胃窦)、TNM分期与CLIC1 表达均是胃癌的预后不良的独立影响因素(均P＜0.01).[结论]CLIC1 可能在胃癌的发生发展过程中发挥重要作用,CLIC1 蛋白的高表达与胃癌的侵袭转移密切相关.“,”[Objective]To investigate the relationship of chloride intracellularchannel 1 expression (CLIC1)with invasion,metastasis and prognosis of gastric cancer (GC).[Methods]The GC tissues and adjacent tissues were obtained from 94 patients.The expression of CLIC1 mRNA and protein were detected.The correlation of CLIC1 protein expression with gastric cancer clinical characteristics was analyzed.Multivariate Cox proportional model was applied to reveal the influencing factors of poor prognosis of gastric cancer.[Results]The positive expression rate of CLIC1 protein in GC adjacent tissues was 25.0%(23／94),which was significantly lower than that of the GC tissues 86%(81／94)(P ＜0.01).The positive expression of CLIC1 was not correlated with age,gender,tumor metastasis,however,it was related with tumor differentiation, lymph node metastasis,depth of invasion and TNM stage (all P ＜0.05).The expression level of CLIC1 mRNA in gastric cancer tissues was significantly higher than that in adjacent normal tissues (P＜0.05).The median survival time of patients with low expression of CLIC1 protein was over 29 months,which was signifi cantly longer than that of patients with positive expression for 25 months (P＜0.05).The 5year survival rate of patients with low expression of CLIC1 protein was 63.9%,which was significantly higher than 20.7% of patients with positive expression of CLIC1 protein (P ＜0.05).GC invasion depth,lymph node metastasis, TNM stage and CLIC1 protein expression were independent prognostic factors for gastric cancer survivals (P＜0.01).[Conclusion]CLIC1 plays an important role in the genesis and development of gastric cancer.High expression of CLIC1 is closely correlated with the invasion and metastasis of GC.