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很多文章曾报道低温预处理可以明显提高大麦花药反应频率。但对于低温预处理的机理，至今研究报道甚少。我们应用ＥＬＩＳＡ方法测定了大麦花药低温预处理过程中内源激素ＩＡＡ、ｉＰＡ和ＡＢＡ含量。根据实验结果，推测低温预处理改变了花药内源ＩＡＡ和ｉＰＡ含量，阻断了花粉原来的发育方向，使其由配子体的发育途径转向孢子体的发育途径。
Many articles have reported that cold pretreatment can significantly increase the frequency of anther response in barley. However, low temperature pretreatment mechanism, so far little research reports. We measured the contents of endogenous hormones IAA, iPA and ABA during barley anther pre-treatment by ELISA. According to the experimental results, we hypothesize that low temperature pretreatment changed the contents of endogenous IAA and iPA in anthers, blocked the original developmental direction of pollen, and shifted the development of gametophyte to sporophyte development.