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菌根菌丝桥是植物间在地下进行物质交流的通道,但它能否作为植物间地下化学通讯的通道来传递抗病信号则缺乏研究.本文利用丛枝菌根真菌(AMF)摩西球囊霉在供体与受体番茄植株间建立菌丝桥,对供体植株接种早疫病病原菌茄链格孢菌,研究供体与受体番茄植株根系间是否存在抗病信号的传递.荧光定量PCR检测表明,AMF侵染后的供体番茄植株再接种病原菌,其根系中苯丙氨酸解氨酶基因(PAL)、脂氧合酶基因(LOX)和几丁质酶基因(PR3)的转录水平显著高于仅接种病原菌、未接种病原菌和AMF,以及只接种AMF的番茄植株.更重要的是,与供体有菌丝桥连接的受体番茄根系中PAL、LOX和PR3的基因的表达量也显著高于无菌丝桥连接、菌丝桥连接被阻断以及有菌丝桥连接但供体植物未接种病原菌的处理,3个基因最高转录水平达到无菌丝桥连接对照受体植物的4.2、4.5和3.5倍.此外,供体植株根系启动防御反应的时间(18和65h)比受体(100和140h)早.表明病原菌诱导番茄供体根系产生的抗病信号可以通过菌丝桥传递到受体根系.
Mycorrhizal hyphae bridge is a pathway for plants to exchange material underground, but whether it can transmit disease-resistant signal as an underground chemical communication channel between plant plants is lack of research.In this paper, AMF Morse balloon The mycelia bridge between donor and recipient tomato plants was inoculated, the donor plants were inoculated with Alternaria solani, the pathogen of Alternaria solani, and the existence of disease-resistance signal transmission between the donor and recipient tomato plants was investigated. The results showed that AMF-infected donor tomato plants were re-inoculated with pathogens, and the transcripts of the PAL, LOX and PR3 genes in the roots of AMF- Levels were significantly higher than those of inoculated pathogen, unvaccinated pathogen and AMF, and AMF-inoculated tomato plants.More importantly, the expression of PAL, LOX and PR3 genes in the recipient tomato root system with donor mycelial bridges The results showed that the maximum transcript level of the three genes reached the highest level at asepsis bridging control recipient plant, which was significantly higher than that of the sterile silk bridge, the mycelium bridge was blocked and the mycelial bridge was connected but the donor plants were not inoculated with pathogens. 4.2, 4.5 and 3.5 times Roots of somatic plants initiated the defensive response time (18 and 65 h) earlier than the recipients (100 and 140 h), indicating that pathogen-induced resistance signals from tomato donor roots can be transmitted through the mycelial bridge to the recipient root.