作者从一例已被职业史,胸片和病理学证实有电焊工尘肺的58岁电焊工尸体上取得的肺组织,经过组织学制备及扫描电子显微镜标本制备,然后用光学显微镜及扫描电子显微镜(SEM)进行检查。肺组织是用10％缓冲的福尔马林液固定,石腊包埋切片和染色,弹性硬蛋白用苏木紫和伊红,范吉逊氏染色,组织学制备是用普鲁士兰铁染色。SEM标本制备是从包埋块上切取两片5微米厚度的石腊切片,分别裱在显微镜载物玻璃片上和2毫米厚的碳质圆片上,用液体二氧化碳置于临界点脱腊并干燥,把裱片粘到铝柱上,用脱水碳包裹,然后 The lung tissue obtained from a 58-year-old welder body that had been proved by occupational history, chest radiography and pathology to have a welder’s pneumoconiosis was histologically prepared and prepared by scanning electron microscopy and then examined by light microscopy and scanning electron microscopy SEM) inspection. Lung tissue was fixed with 10% buffered formalin, embedded in paraffin and stained with paraffin, stained with hematoxylin and eosin, van Giessen, histologically stained with Prussian blue iron. SEM specimens were prepared by cutting two pieces of paraffin slices with a thickness of 5 micrometers from the embedding blocks, mounting them on a microscope carrier glass piece and a 2 mm-thick carbon disc respectively, de-waxing and drying with liquid carbon dioxide at a critical point, Stick the mounting plate to the aluminum column, wrap it with dehydrated carbon, and then