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突变体的创建和筛选是挖掘基因功能的重要来源,也是扩充种质资源的有效途径之一。利用60Coγ射线处理籼稻“9311”种子,经过M2筛选及M3鉴定,共获得128份突变体,突变频率为10.24%。突变类型包括生育期及育性突变、叶片性状突变、茎秆形态突变、穗部形态突变、子粒形态突变等,此外,还发现一个植株变矮、节间和叶片变短且不能进入生殖生长的突变体,该突变体在湖北和海南连续种植都不能开花结实。筛选的突变体不仅为水稻功能基因组学研究提供丰富的材料,部分突变体还可直接作为育种材料应用。
The creation and screening of mutants is an important source of mining gene function and an effective way to expand germplasm resources. A total of 128 mutants were obtained by treatment of 60Coγ-ray seeds of indica rice “9311” with M2 screening and identification of M3. The frequency of mutation was 10.24%. The types of mutation include the growth and fertility mutation, the leaf trait mutation, the stalk morphological change, the spike morphological mutation, the mutation of the grain morphology and the like. In addition, one plant was found to be shorter, the internode and the leaf blade shorter and unable to enter the reproductive growth Mutant, the mutant in Hubei and Hainan continuous planting can not flowering and fruiting. Screening mutants not only provide abundant materials for functional genomics research in rice, but also some mutants can be directly used as breeding materials.