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以土壤农杆菌为介导，用叶盘法转化获得的转ＣＭＶ－ＣＰ基因（黄瓜花叶病毒外壳蛋白基因）番茄工程植株，通过连续选择和自交，得到Ｒ１、Ｒ２、Ｒ３、Ｒ４和Ｒ５代的转化番茄植株。１９９３～１９９４年采用人工接种和田间自然发病两种方式鉴定转化植株对ＣＭＶ病毒的田间抗性。结果表明，转基因番茄植株对ＣＭＶ侵染有高度抗性。人工接种Ｒ１、Ｒ２、Ｒ３、Ｒ４代转化植株的防病效果达５０％～７３％。有５０％以上的转基因植株始终未被ＣＭＶ病毒侵染，表现为免疫抗性。自然条件下，Ｒ２～Ｒ５代转化植株的防病效果达５７％～７９％。转化植株的产量比对照人工接种的高１０～６０倍，比自然发病的增加５倍以上
The Agrobacterium tumefaciens-mediated tomato plants transformed with the CMV-CP gene (cucumber mosaic virus coat protein gene) were obtained by leaf disk method and were obtained by continuous selection and selfing to obtain R1, R2, R3, R4 and R5 Generation tomato plants. From 1993 to 1994, two methods of artificial inoculation and natural occurrence in the field were used to identify the resistance of the transformed plants to CMV in the field. The results showed that transgenic tomato plants were highly resistant to CMV infection. Artificially inoculated R1, R2, R3, R4 on behalf of the plant transformation control effect of 50% to 73%. More than 50% of the transgenic plants have never been infected with CMV virus, showing immune resistance. Under natural conditions, the disease control effect of R2 ~ R5 generation plants reached 57% ~ 79%. The yield of the transformed plants was 10 to 60 times higher than that of the control inoculation and more than 5 times more than the natural incidence