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目的探索用腺病毒作载体对脑胶质瘤进行基因治疗。方法构建含CMV启动子的HSV-tk基因的重组腺病毒载体,将HSV-tk基因转移至人脑胶质瘤细胞SHG_(44),用MTT方法测定GCV对肿瘤细胞的杀伤效果。结果转染HSV-tk基因的SHG_(44)细胞对GCV的敏感性增高80倍,MOT为100时,5mg/L的GCV能完全杀死肿瘤细胞,并存在旁观者效应,一个转基因细胞能引起10倍的亲本细胞对GCV敏感。结论腺病毒介导的HSV-tk/GCV系统能有效杀伤人脑胶质瘤细胞。
Objective To explore adenovirus vector for gene therapy of glioma. Methods HSV-tk gene containing CMV promoter was constructed and transfected into human glioma cell line SHG_ (44). The killing effect of GCV on tumor cells was determined by MTT assay. Results The sensitivity of HSV-tk gene transfected SHG 44 cells to GCV was increased 80-fold. When MOT was 100, 5 mg / L GCV completely killed tumor cells and there was a bystander effect. One transgenic cell could cause Ten times more parental cells are sensitive to GCV. Conclusions Adenovirus-mediated HSV-tk / GCV system can effectively kill human glioma cells.