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目的建立多伞阿魏药材的GC-MS指纹图谱。方法采用水蒸气蒸馏法提取8个产地44份多伞阿魏挥发油,通过GC-MS分析其成分。运用中药色谱指纹图谱相似度建立共有模式,以2种方法计算相似度评价图谱的相似性,同时利用聚类分析法和主成分分析法分析结果。结果建立了多伞阿魏挥发油GC-MS指纹图谱分析方法;对44份多伞阿魏挥发油样品进行了分析,确立了12个共有峰。GC-MS指纹图谱的相似度>0.90的有37个。44份多伞阿魏药材的挥发油可通过系统聚类归为2类,该结果与指纹图谱的相似度分析结果非常相似,不同产地多伞阿魏药材的挥发油相似度较高。主成分分析表明愈创木醇在多伞阿魏挥发油的质量控制中起着比较重要的作用。结论该方法简便、准确、重现性好,可用于多伞阿魏药材的质量综合评价。
Objective To establish the GC-MS fingerprinting of Radix Euphorbiae medicinal materials. Methods Steam distillation was used to extract 44 volatile oils from 8 producing areas, and their compositions were analyzed by GC-MS. The common mode was established by using the similarity of chromatographic fingerprints of traditional Chinese medicines. The similarity of the similarity evaluation maps was calculated by two methods, and the results were analyzed by cluster analysis and principal component analysis. Results A GC-MS fingerprinting method was established for the volatile oil from Radix Euphratica. 44 samples of volatile oil from Radix Euphorbiae were analyzed, and 12 common peaks were established. The similarity of GC-MS fingerprints was> 0.90 and 37. The volatile oils from 44 medicinal materials of Radix Ulextillae can be classified into two categories by systematic clustering. The results of similarity analysis of the results with the fingerprints are very similar, and the similarity of the volatile oils of Radix Euphorbiae medicinal materials is high. Principal component analysis showed that guaifenesol played a more important role in the quality control of volatile oil from Radix Euphorbiae. Conclusion The method is simple, accurate and reproducible, and can be used for the comprehensive quality evaluation of Radix Euphorbiae medicinal materials.