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: [目的 ]开展 HIV- 1血浆病毒载量 (VL )的检测 ,探讨我省不同临床阶段 HIV感染者血浆 VL水平与其临床表现、预后之间的关系。 [方法 ]定期随访我省 1992～ 1999年发现的部分无症状 HIV感染者和有艾滋病相关综合征 /艾滋病的病人 (各 4例 ) ,并采集外周血。应用等温核酸序列扩增技术 (NASBA)测定各病例的血浆 HIV- 1病毒载量(VL )。在随访中 1例出现临床进展 ,进行了第 2次 VL测定 (间隔 6个月 )。 [结果 ]不同临床阶段的 HIV感染者 8例(9份血浆 )全部检出 HIV- 1RNA,敏感性达 10 0 %。有症状 HIV感染者的 VL显著高于无症状感染者 ,分别为(4 .890± 0 .336 ) lg/ ml和 (3.6 18± 0 .2 0 6 ) lg/ ml。在随访期间出现临床进展者前后两次的 VL分别为 3.778lg/ ml和4.5 31lg/ ml,升高 0 .75 3 lg。 [结论 ]上述 8例我省不同临床阶段的 HIV感染者体内均存在活跃的 HIV病毒复制 ,但其复制水平可能与感染者的临床阶段和预后有一定关系
: [Objective] To detect the HIV-1 plasma viral load (VL) and to explore the relationship between plasma VL levels and clinical manifestations and prognosis in HIV-infected patients at different clinical stages in our province. [Methods] Regular follow-up of some of the asymptomatic HIV-infected persons and AIDS-related syndrome / AIDS patients (4 in each) found in our province from 1992 to 1999, and peripheral blood was collected. Plasma HIV-1 viral load (VL) was determined for each case using isothermal nucleic acid sequence amplification (NASBA). One patient developed clinical follow-up and performed the second VL measurement (interval 6 months). [Results] HIV-1 RNA was detected in 8 cases (9 plasma) in all clinical stages of HIV infection and the sensitivity was 100%. The VL of patients with symptomatic HIV infection was significantly higher than that of asymptomatic patients (4.90 ± 0.336) gg / ml and (3.618 ± 0.260) gg / ml, respectively. The VLs before and after clinical progression during follow-up were 3.778 lg / ml and 4.531 lg / ml, respectively, an increase of 0.753 lg. [Conclusion] The above 8 cases of HIV infection in different clinical stages of our province exist active HIV virus replication, but the level of replication may be related to the clinical stage and prognosis of infected patients have a certain relationship