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为探讨细胞因子信号转导抑制因子-3(SOCS-3)和肿瘤坏死因子-α(TNF-α)mRNA表达在毒鼠强中毒(TEMP)致多器官功能衰竭(MODS)中的作用,将30只健康雄性BALB/c小鼠随机分成TEMP模型组(n=10)、生理盐水对照组(n=10)、正常对照组(n=10),分别于中毒后2、6、12、24 h取小鼠的肝脏,采用逆转录聚合酶链反应(RT-PCR)测定肝组织SOCS-3和TNF-αmRNA表达水平。结果显示,中毒后2、6、12和24 h肝组织中SOCS-3 mRNA表达量均明显高于对照组(P均<0.05),24 h达到峰值。TNF-αmRNA表达量均明显高于对照组(P均<0.05),12 h达峰值。相关分析显示,肝脏中SOCS-3 mRNA与TNF-αmRNA表达存在明显的正相关性(y=0.091+0.747x,r=0.912,F=261.2,P<0.01)。提示不同时间点TEM中毒小鼠肝脏中SOCS-3、TNF-αmRNA均升高且表达趋势一致。
To investigate the role of SOCS-3 and TNF-α mRNA expression in multiple organ failure (MODS) caused by tetramine poisoning (TEMP), we investigated the effects of SOCS-3 and TNF- Thirty healthy male BALB / c mice were randomly divided into TEMP model group (n = 10), normal saline control group (n = 10) and normal control group (n = 10) h, the liver of mice was taken and the expression of SOCS-3 and TNF-αmRNA in liver tissue was determined by reverse transcription-polymerase chain reaction (RT-PCR). The results showed that at 2, 6, 12 and 24 h after poisoning, the expression of SOCS-3 mRNA in liver tissue was significantly higher than that in the control group (all P <0.05), reaching the peak at 24 h. TNF-αmRNA expression levels were significantly higher than the control group (P <0.05), peaked at 12 h. Correlation analysis showed that there was a significant positive correlation between SOCS-3 mRNA and TNF-αmRNA in the liver (y = 0.091 + 0.747x, r = 0.912, F = 261.2, P <0.01). These results suggest that SOCS-3 and TNF-αmRNA in the liver of TEM poisoning mice were all increased at different time points and the expression trends were consistent.