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目的:为了进一步了解丙型肝炎病毒(HCV)的感染者血清中HCV的存在情况、结合其它指标,可以判定丙型肝炎病人的病情是何种情况,以指导临床的治疗与监测。方法:应用酶联免疫吸附试验(ELISA)方法测定抗—HCV:应用聚合权衡利弊链反应(PCR)检测HCV—RNA。结果:2470例住院患者检测抗—HCV,有80例阳性,其中患血液病的患者抗—HCV阳性为28例,占总阳性35%;患肝病的抗—HCV阳性为18例,占总阳性22.5%:透析患者抗—HCV阳性为8例、占总阳性10%:其它疾病有输血史的12例抗—HCV阳性,占总阳性15%;其它疾病无输血史14例抗—HCV阳性,占总阳性17.5%。对20例抗—HCV阳性患者应用PCR方法检测HCV—RNA,其中17例阳性,阳性率为85%。结论:通过测定结果的分析,可以看出输血是HCV的重要传染途径。阻断通过血源传播HCV是我们医务工作者面临的一个非常重要的课题。
Objectives: In order to further understand the presence of HCV in the serum of patients infected with hepatitis C virus and other indicators, we can determine the condition of patients with hepatitis C to guide the clinical treatment and monitoring. Methods: Anti-HCV was determined by enzyme linked immunosorbent assay (ELISA): HCV-RNA was detected by polymerase chain reaction (PCR). Results: 2470 inpatients were tested for anti-HCV, 80 were positive, of which 28 were anti-HCV positive in patients with hematological disease, accounting for 35% of the total; 18 were anti-HCV positive for liver disease, 22.5%: dialysis patients were anti-HCV positive in 8 cases, accounting for 10% of the total positive: other diseases with blood transfusion history of 12 anti-HCV positive, accounting for 15% of the total positive; no other blood transfusion history of 14 anti-HCV positive, The total positive 17.5%. HCV-RNA was detected by PCR in 20 anti-HCV positive patients, of which 17 were positive and the positive rate was 85%. Conclusion: The results of the analysis, we can see that blood transfusion is an important route of transmission of HCV. Blocking blood-borne HCV is a very important issue for our healthcare workers.