论文部分内容阅读
本文证明琥珀酸细胞色素c还原酶中细胞色素b的还原,无论用琥珀酸还是用四甲基氢醌作底物,在pH6.0—9.0范围内均呈现出三相过程。从pH6.0到pH8.0,细胞色素b的初相还原速度都快于细胞色素c_1的还原速度。b的各相速度和c_1的还原速度都随着pH增加而增加。从细胞色素b的三相变化过程和加入外源细胞色素c后的多相氧化还原过程的低温差光谱反复扫描实验中,可清楚地看到b在562nm吸收峰的出现及消失,表明了b的还原或氧化。这些结果与单波长562nm监测的结果是一致的。
This paper demonstrates that the reduction of cytochrome b in succinic acid cytochrome c reductase shows a three-phase process in the range of pH 6.0-9.0, both with succinic acid and tetramethylhydroquinone as substrates. From pH 6.0 to pH 8.0, the initial reduction rate of cytochrome b was faster than that of cytochrome c_1. The phase velocity of b and the reduction rate of c_1 both increase with increasing pH. The appearance and disappearance of the absorption peak of b at 562 nm can clearly be seen from the experiments of three-phase change of cytochrome b and the low temperature difference spectrum repeated-phase analysis of the heterogeneous redox process after addition of exogenous cytochrome c, indicating that b Reduction or oxidation. These results are consistent with the results of a single wavelength 562 nm monitoring.