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重组人促红细胞生成素（rHuEPO）是一种复杂的糖蛋白药物，含有三个氮连接和一个氧连接的糖基化位点。肽图谱分析是糖蛋白分析的有效手段，可是由于糖基化的影响，不是所有理论酶解位点都酶解了，需要实验验证。本文采用谷氨酸内切酶(Glu-C)将rHuEPO所有糖基化位点酶解成含糖肽段，HPLC/ESI/MS肽图谱分析，并通过源内碰撞诱导解离（In-source CID）分析了氨基酸序列。理论肽段有7段，检出了6段，只有一个三肽未检出。实验得到了含双硫键的肽段，通过CID分析确证了小分子肽段的氨基酸序列。
Recombinant human erythropoietin (rHuEPO) is a complex glycoprotein drug containing three nitrogen-linked and one oxygen-linked glycosylation sites. Peptide profiling is an effective means of glycoprotein analysis, but not all theoretical proteolytic sites are enzymatically digested due to glycosylation, and experimental validation is needed. In this paper, all glycosylation sites of rHuEPO were enzymatically digested into glycopeptide fragments by HPLC using glutamate endonuclease (Glu-C), HPLC / ESI / MS peptide mapping and in-source CID ) Analyzed the amino acid sequence. The theoretical peptide has 7 segments, 6 segments have been detected, and only one tripeptide has not been detected. The disulfide bond-containing peptides were obtained by experiments. The amino acid sequences of small peptides were confirmed by CID analysis.