Objective:To evaluate the antibacterial activity of the methanolic extract of mango (Mangifera indicaL.) seed kel.Methods: Chokanan mango seed kel and seed kels from assorted mango varieties were collected, cleaned, dried and powered. Crude methanolic extracts of mango seed kel were analyzed for the phytochemical constituents. The free radical scavenging activity was determined by2,2-Diphenyl-1-picrylhydrazyl(DPPH)assay. Antibacterial activity was evaluated by disc diffusion assay with three medically important bacterial pathogens such as methicillin resistantStaphylococcus aureus (S. aureus)(MRSA),Escherichia coli (E. coli) andVibrio vulnificus (V. vulnificus).Results: Qualitative phytochemical analysis indicated the presence of important phytochemical compounds such as glycosides, saponins, flavanoids, tannins and alkaloids. There was no significant difference in the phytochemical content between the single and assorted mango seed kels. However, the free radical scavenging study indicated that the assorted mango kels showed slightly higher activity than the single species (P<0.05). The crude methanolic extract of mango seed kel at a concentration of100 mg/mL is found to have potential antimicrobial activity againstMRSA and E. coli compared toV. vulnificus. Study on the antibacterial activity also indicated that there was no significant difference in the antibacterial activity of the single and assorted mango seed kel extracts.Conclusions: The present study conclusively demonstrates the free radical scavenging activity and antibacterial activities of mango seed kel. In addition, the results also indicated that there is no significant difference in the phytochemical content and biological activity of mango kels from single and assorted mango varieties.