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Background/Aims: The role of antibody dependent cellular cytotoxicity (ADCC) i n HCV infection is unclear at prese- nt.Antibodies mediating ADCC are usually directed against viral envelope prote ins. As cell surface expression of the HCV envelope E2 protein has been shown, t he HCV E2 protein is an especially promising candidate target for ADCC. Methods:Sera fro m patients with acute (n=6), self-limited (n=11) and chronic (n=19) HCV infecti on were analyzed in this study.Sera reacting with cell-bound HCV antigens were examined in a flowcytometric cytotoxicity assay using antigen-coated JOK-1 cel ls as targets. Results: We found that sera from all stages of HCV infection reac ted with cells loaded with HCV E2. E2-specific ADCC was observed in patients wi th acute (n=3/6),self-limited (n=5/11) and chronic (n=13/19) hepatitis C and wa s closely related to fluorescence intensity in the E2-binding assay (r=0.67, P< 0.001). Conclusions: We conclude that E2-antibodies from all stages of HCV infe ction can mediate ADCC.Thus, the role of this process in the pathogenesis of chr onic hepatitis C should be further elucidated.
Background / Aims: The role of antibody dependent cellular cytotoxicity (ADCC) in HCV infection is unclear at prese-nt. Antibodies mediating ADCC are usually directed against viral envelope prote ins. As cell surface expression of the HCV envelope E2 protein has been shown, Methods: Sera fro m patients with acute (n = 6), self-limited (n = 11) and chronic (n = 19) HCV infecti on were analyzed in this study. Rapid with cell-bound HCV antigens were examined in a flowcytometric cytotoxicity assay using antigen-coated JOK-1 cel ls as targets. Results: We found that sera from all stages of HCV infection reac ted with cells loaded with HCV E2. E2-specific ADCC was observed in patients wi th acute (n = 3/6), self-limited (n = 5/11) and chronic (n = 13/19) hepatitis C and wa s closely related to fluorescence intensity in the E2-binding assay (r = 0.67, P <0.001) Conclusions: We conclude that E2-antibodies from all stages of HCV i nfe ction can mediate ADCC.Thus, the role of this process in the pathogenesis of chr onic hepatitis C should be further elucidated.