论文部分内容阅读
【目的】研究多粘类芽孢杆菌(Paenibacillus polymyxa)CP7菌株的抗菌蛋白(CP7ACP)对嗜水气单胞菌的抑杀作用机理,为防治嗜水气单胞菌引起的鱼病提供新的潜在天然药物。【方法】采用抑菌试验、钼锑抗比色法和紫外光谱法研究其对嗜水气单胞菌S12菌株生长、磷泄漏和生物大分子的影响,并利用扫描电镜和透射电镜观察了嗜水气单胞菌细胞结构遭受的破坏作用。【结果】CP7ACP对嗜水气单胞菌的抑菌圈直径约8.1 mm,最小抑菌浓度(MIC)与最小杀菌浓度(MBC)分别为原液浓度的1/8和1/4;嗜水气单胞菌受CP7ACP处理后,电镜观察发现其细胞壁、细胞膜、细胞器以及菌体均受到不同程度的破坏,胞内的生物大分子和磷泄漏明显,基因组DNA发生增色效应。【结论】CP7ACP抑制嗜水气单胞菌生长,可用于防治嗜水气单胞菌引起的鱼病。
【Objective】 The objective of this study was to investigate the mechanism of inhibition of Aeromonas hydrophila by antimicrobial protein (CP7ACP) from Paenibacillus polymyxa strain CP7 and to provide new potential for controlling the disease caused by Aeromonas hydrophila natural medicine. 【Method】 The effects of bacteriostasis, molybdenum antimony colorimetry and ultraviolet spectroscopy on the growth, phosphorus leakage and biomacromolecules of Aeromonas hydrophila S12 were studied. The results of scanning electron microscopy and transmission electron microscopy Aquatic Aeromonas cell structure suffered damage. 【Result】 The inhibition zone diameter of CP7ACP against Aeromonas hydrophila was about 8.1 mm and the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were 1/8 and 1/4 of that of the stock solution respectively. After treated with CP7ACP, the cell wall, cell membrane, organelles and cell bodies were all damaged by CP7ACP. The leakage of biological macromolecules and phosphorus in the cells was obvious, and the genomic DNA was hyperchromic. 【Conclusion】 CP7ACP can inhibit the growth of Aeromonas hydrophila and it can be used to control the fish disease caused by Aeromonas hydrophila.